Police, fire and ambulance were called to the scene, 80km east of the Stuart Highway intersection, shortly before 5pm after a member of the public reported the incident.

Upon arrival, police reported a head-on collision between a truck and a sedan.

A 25-year-old female passenger and an infant child were reported dead at the scene despite the best efforts of witnesses and first responders.

A 24-year-old woman was rescued from the vehicle and was airlifted along with the 22-year-old male driver to Royal Darwin Hospital.

The truck driver was uninjured and both drivers tested negative for alcohol.

The road remained closed while Major Crash detectives continued their investigation.